DNA

Part:BBa_K4438600

Designed by: Neha Adarsh   Group: iGEM22_IISER-Tirupati_India   (2022-10-11)

Aptamer_T6

Aptamer_T6 is a single-stranded DNA aptamer having 86 nucleotides. Figure 1A) Shows the secondary structure of the aptamer and its minimum free energy.

Usage and Biology

This aptamer has a high affinity for testosterone with KD being 1.8 ± 0.3 nM. This was calculated using Apta-PCR affinity assay (APAA). This part can be used to develop aptasensors that can detect different concentrations of testosterone in biological samples [1]. T--IISER-Tirupati India--T6 2.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 31
    Illegal SpeI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 31
    Illegal SpeI site found at 67
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 31
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 31
    Illegal SpeI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 31
    Illegal SpeI site found at 67
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.

[edit]
Categories
Parameters
None